Fig 1: SYNCRIP-deficiency confers resistance to AR-targeted therapies(A) Stacked bar plot represents the percentage of PCa samples with genomic alterations of SYNCRIP, created using cbioportal.org.(B) Kaplan-Meier curve represents the treatment duration on AR-targeted therapies of patients with mCRPC patients (WT n = 50, Hom-del n = 9). Abi: abiraterone, Enz: enzalutamide, Apa: apalutamide. p value was calculated with Mantel-Cox test.(C) Cox proportional hazard ratio analysis of this cohort, p value was calculated with Log rank test.(D) Bar plot represents the percentage of PSA change in 12 weeks in patients receiving AR-targeted therapies, p value was calculated with two-tailed Mann-Whitney test.(E) Western blot of SYNCRIP and AR in a series of human PCa cell lines.(F–H) Bar plots represent the relative cell viability of LNCaP/AR (F), relative cell number fold change of CWR22Pc (G), and MDA-PCa-2b (H) cells transduced with annotated guide RNAs, and/or rescue plasmids (SYN_Res), measured as values of relative luminescence unit (RLU) or cell number fold change and normalized to vehicle conditions. Enz denotes 10 µM enzalutamide for LNCaP/AR, 1 µM for CWR22Pc, 5 µM for MDA-PCa-2b for 7 days and Veh denotes DMSO.(I) Cell number of LNCaP/AR cells transduced with annotated guide RNAs, and/or rescue plasmids (SYN_Res), measured by cell proliferation assay. Enz denotes 10 µM enzalutamide treatment.(J) Tumor growth curve of xenografted LNCaP/AR cells transduced with annotated guide RNAs in castrated mice. Enz denotes enzalutamide at 10 mg/kg. A number of tumors were annotated.(K) H&E and IHC staining on sgNT and sgSYNCRIP xenografts tumor slides.For all panels unless otherwise noted, 3 biological replicates in each group and mean ± s.e.m. is represented. p values were calculated using two-way ANOVA with Bonferroni multiple-comparison test. All schematic figures were created with BioRender.com. See also Figures S1, S2, and Table S1.
Fig 2: SYNCRIP-deficiency unleashes ectopic APOBEC-driven DNA mutagenesis(A) Relative expression of AR-targeted genes in LNCaP/AR cells transduced with annotated guide RNAs and treated with vehicle (Veh, DMSO) or enzalutamide (Enz, 10 µM).(B and C) Representative image and quantification of IHC staining in sgNT and sgSYNCRIP xenograft tumors.(D–E) Bar plot represents the counts of C?T/G/A mutations in TCW motif (D) and APOBEC-signature mutations (E) in LNCaP/AR cells transduced with annotated shRNAs. p values were calculated using one-way ANOVA with Bonferroni multiple-comparison test.(F) Schematic figure illustrates SKSC or NTSC generation.(G) Bar plots represent the APOBEC signature mutations in single clones derived from SYNCRIP-deficient cells (SKSCs) and wild-type cells (NTSCs).(H) Bar plot represents the percentage of each SBS signatures identified in the SKSCs and NTSCs.(I) Schematic figure represents the FRET-based APOBEC deaminase activity assay.(J) Bar plot represents the relative fluorescence unit (RFU) of APOBEC deaminase activity in serially diluted cell extracts from LNCaP/AR cells transduced with annotated guide RNAs.For all panels unless otherwise noted, 3 biological replicates in each group and mean ± s.e.m. is represented. p values were calculated using two-way ANOVA with Bonferroni multiple-comparison test. Schematic figures were created with BioRender.com. See also Figure S3, and Table S2.
Fig 3: Evolutionary trajectory analysis revealed the dominant-resistant subclones with FOXA1 mutations(A) UMAP plots represent the pseudotime trajectory of single cells of the SYNCRIP-deficient (shSYN) clusters. Color intensity represents the pseudotime estimation (B) UMAP plot represents the 7 SYNCRIP-deficient (shSYN) clusters of the single cell.(C) UMAP plot represents the APOBEC-caused mutation counts per cell.(D) UMAP plot represents the ITH score. For panels A–D, arrows and dot line represents the direction of pseudotime flow.(E) Heatmap represents the APOBEC-caused mutations (normalized mutation counts/cell) within 8 resistance drivers. Winner clusters were presented in green and loser clusters were presented in red.(F–H) Heatmap represents the expression of FOXA1 (F) and AR (G, H) downstream genes in each single cell along the pseudotime trajectory. For panels F–H, pseudotime estimation, normalized gene expression, and clusters are presented with different colored bars. Cluster 3 was annotated with blue square.(I) Schematic figure represents the hypothetic model, created with BioRender.com.See also Figure S8 and Table S7.
Fig 4: SREX-resistant cell lines revealed high tumor heterogeneity in SYNCRIP-deficient tumors(A) Schematic representation of the generation of SREX and NREX lines, created with BioRender.com.(B) Heatmap represents the expression fold changes (log10) of top 8 APOBEC-mutated resistance driver genes in a panel of SREX and NREX lines.(C) Heatmap represents the expression fold changes (log10) of top selected downstream targeted genes in SREX and NREX lines.(D) Heatmap represents the expression fold changes (log10) of marker genes for lineage-related transcriptional programs in SREX and NREX lines. For panels (B–D), results of qPCR assays and the average expression fold change (log10) of 3 biological replicates are shown.(E) Heatmap represents the normalized mutation counts (WES) in driver gene in SREX lines.(F–M) Relative cell number fold change of selected SREX lines treated with 10 µM enzalutamide (Enz), measured by cell proliferation assay and normalized to sgNT. For panel (F–M), 3 biological replicates in each group and mean ± s.e.m. is represented, p values were calculated using one-way ANOVA with Bonferroni multiple-comparison test.See also Figure S7 and Table S6.
Fig 5: APOBEC3B-driven mutagenesis is required and sufficient to promote resistance(A) Co-IP of SYNCRIP and APOBECs in HEK293T cells.(B) Co-IP of endogenous SYNCRIP and APOBEC3B in LNCaP/AR cells.(C) Co-IP of endogenous SYNCRIP and flag-tagged APOBEC3B in LNCaP/AR cells.(D) Co-IP of endogenous APOBEC3B and flag-tagged SYNCRIP in LNCaP/AR cells.(E) Bar plot represents the relative cell number fold change of LNCaP/AR cells transduced with annotated guide RNAs and shRNAs.(F) IF staining of annotated endogenous APOBECs and SYNCRIP in LNCaP/AR cells.(G) Bar plot represents the fold change of APOBEC-driven mutations observed in RTCW and YTCW motifs.(H) Bar plot represents the RFU of APOBEC deaminase activity in LNCaP/AR cells. p values were calculated using one-way ANOVA with Bonferroni multiple-comparison test.(I) Bar plot represents the relative fold change in cell numbers of sgNT or sgSYNCRIP LNCaP/AR cells, treated with Veh, Enz, or a combination of Enz and two A3B inhibitors (1 µM phloretin, 10 µM icariside I).(J) Bar plot represents the relative cell number fold change of LNCaP/AR cells transduced with constructs expressing annotated proteins.(K) Fluorescence pictures represent comet assay of LNCaP/AR cells transduced with sgNT, sgSYNCRIP, and A3B-OE constructs.(L) Co-IP of HA-tagged SYNCRIP and Flag-tagged APOBEC3B, APOBEC3B N-terminus and C-terminus regions in HEK293T cells.(M) Bar plot represents the relative cell number fold change of LNCaP/AR cells transduced with annotated guide RNAs, and/or rescue plasmids, measured by cell proliferation assay.For all panels unless otherwise noted, 3 biological replicates in each group and mean ± s.e.m. is represented. p values were calculated using two-way ANOVA with Bonferroni multiple-comparison test. Enz denotes 10 µM enzalutamide and Veh denotes DMSO. See also Figures S3 and S4.
Supplier Page from MilliporeSigma for Anti-SYNCRIP antibody produced in rabbit